CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F14%3A00061422" target="_blank" >RIV/00159816:_____/14:00061422 - isvavai.cz</a>

Alternative codes found

RIV/68081715:_____/14:00432067 RIV/00216224:14110/14:00073246

Result on the web

<a href="http://dx.doi.org/10.1007/s00216-014-8053-8" target="_blank" >http://dx.doi.org/10.1007/s00216-014-8053-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-014-8053-8" target="_blank" >10.1007/s00216-014-8053-8</a>

Result language

angličtina

Original language name

CIEF separation, UV detection, and quantification of ampholytic antibiotics and bacteria from different matrices

Original language description

The effect of antibiotics on the microbial cells and concentration of antibiotics in the human body is essential for the effective use of antimicrobial therapy. The capillary isoelectric focusing is a suitable technique for the separation and the detection of bacteria, and amphoteric substances from nature. However, the determination of isoelectric points of ampholytic antibiotics by conventional techniques is time consuming. For this reason, capillary isoelectric focusing seems to be appropriate as a simple and reliable way for establishing them. The separation conditions for the capillary isoelectric focusing of selected ampholytic antibiotics with known isoelectric points and pK (a)s, ampicillin (pI 4.9), ciprofloxacin (pI 7.4), ofloxacin (pI 7.1),tetracycline (pI 5.4), tigecycline (pI 9.7), and vancomycin (pI 8.1), were found and optimized in the suitable pH ranges pH 2.0-5.3, 2.0-9.6, and 9.0-10.4. The established values of isoelectric points correspond with those found in the li

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

406

Issue of the periodical within the volume

25

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

6285-6296

UT code for WoS article

000342224300020

EID of the result in the Scopus database

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