Advanced online mass spectrometry detection of proteins separated by capillary isoelectric focusing after sequential injection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12520%2F17%3A43895941" target="_blank" >RIV/60076658:12520/17:43895941 - isvavai.cz</a>

Result on the web

<a href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700695/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/jssc.201700695/epdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201700695" target="_blank" >10.1002/jssc.201700695</a>

Result language

angličtina

Original language name

Advanced online mass spectrometry detection of proteins separated by capillary isoelectric focusing after sequential injection

Original language description

Capillary isoelectric focusing hyphenated with mass spectrometry detection, following the sequential injection of the carrier ampholytes and the sample zone, is highly efficient for the characterization of proteins. The main advantage of the sequential injection protocol is that ampholytes, with pH ranges, which are not supposed to cover the isoelectric points of the sample components, can be used for separation. The method then allows online mass spectrometry detection of separated analytes either in the absence (substances that have left the pH gradient) or in the presence of low-level ampholytes (substances that are migrating within the pH gradient). The appearance of the substances within, or outside the pH gradient depends on, e.g., the composition of the ampholytes (broad or narrow pH range) or on the composition of electrolyte solutions. The experiments performed in coated capillaries (with polyvinyl alcohol or with polyacrylamide) show that the amount and the injection length of the ampholytes influence the length of the pH gradient formed in the capillary.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Volume of the periodical

40

Issue of the periodical within the volume

24

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

4825-4834

UT code for WoS article

000419107900018

EID of the result in the Scopus database

2-s2.0-85034259751