Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F14%3A00061449" target="_blank" >RIV/00159816:_____/14:00061449 - isvavai.cz</a>
Alternative codes found
RIV/68081715:_____/14:00432525 RIV/00216224:14110/14:00073247
Result on the web
<a href="http://dx.doi.org/10.1021/ac502254f" target="_blank" >http://dx.doi.org/10.1021/ac502254f</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/ac502254f" target="_blank" >10.1021/ac502254f</a>

Result language
angličtina
Original language name
Separation of Methicillin-Resistant from Methicillin-Susceptible Staphylococcus aureus by Electrophoretic Methods in Fused Silica Capillaries Etched with Supercritical Water
Original language description
Identification and prevention of Staphylococcus aureus-caused infections may benefit from a fast and dependable method to distinguish between the methicillin-resistant (MRSA) and methicillin susceptible (MSSA) S. aureus strains. The current methods involving polymerase chain reaction and/or other molecular tests are usually laborious and time-consuming. We describe here a fasgt and low-cost method employing capillary zone electrophoresis (CZE) to distinguish between MRSA and MSSA. The method makes use of a supecritical water-treated fused silica capillary, the inner surface of which has subsequently been modified with (3 glycidyloxypropyl)trimethoxysilane. With optimized proportions fo suitable additives to the background electrolyte, a CZE separationof MRSA from MSSA may be completed within 12 min. The cells were baseline-resolved, and resolution was determined to be 3.61. the isoelectric points of MSSA and MRSA were found to be the same for both groups of these strains, pI= 3.4.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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