Comparison of catalysis by haloalkane dehalogenases in aqueous solutions of deep eutectic and organic solvents

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F14%3A00063737" target="_blank" >RIV/00159816:_____/14:00063737 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1039/c4gc00117f" target="_blank" >http://dx.doi.org/10.1039/c4gc00117f</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c4gc00117f" target="_blank" >10.1039/c4gc00117f</a>

Result language

angličtina

Original language name

Comparison of catalysis by haloalkane dehalogenases in aqueous solutions of deep eutectic and organic solvents

Original language description

Haloalkane dehalogenases catalyze the hydrolytic cleavage of carbon-halogen bonds in diverse halogenated hydrocarbons and are attractive catalysts for sustainable biotechnologies. However, their use in industrial processes is limited due to the poor water solubility of their substrates and the tendency of the substrates to undergo abiotic hydrolysis. Here we systematically and critically compare the performance of three haloalkane dehalogenases, DbjA, DhaA and LinB, in aqueous solutions of the deep eutectic solvent ethaline, its components (ethylene glycol and choline chloride), and two organic solvents (methanol and acetone). Each of the solvents had different effects on the activity of each enzyme. Haloalkane dehalogenase DhaA was found to be the most tolerant to ethaline, retaining 21% of its reference activity even in solutions containing 90% (v/v) of ethaline. However, dissolution in 75% (v/v) ethylene glycol, 50% (v/v) methanol, or 25% (v/v) acetone caused almost total loss of Dh

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/ED1.100%2F02%2F0123" target="_blank" >ED1.100/02/0123: St. Anne´s University Hospital Brno - International Clinical Research Center (FNUSA-ICRC)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Green Chemistry

ISSN

1463-9262

e-ISSN

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Volume of the periodical

16

Issue of the periodical within the volume

5

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

2754-2761

UT code for WoS article

000335005200049

EID of the result in the Scopus database

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