Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00065823" target="_blank" >RIV/00216224:14310/13:00065823 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/13:00063741

Result on the web

<a href="http://dx.doi.org/10.1002/biot.201200378" target="_blank" >http://dx.doi.org/10.1002/biot.201200378</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/biot.201200378" target="_blank" >10.1002/biot.201200378</a>

Result language

angličtina

Original language name

Organic Co-solvents Affect Activity, Stability and Enantioselectivity of Haloalkane Dehalogenases

Original language description

Haloalkane dehalogenases are microbial enzymes with a wide range of biotechnological applications. The use of organic co-solvents to solubilize their hydrophobic substrates is often necessary. In order to choose the most compatible co-solvent, the effects of fourteen co-solvents on activity, stability and enantioselectivity of three model enzymes, DbjA, DhaA and LinB, were evaluated. All co-solvents caused at high concentration loss of activity and conformational changes. The highest inactivation was induced by tetrahydrofuran, while more hydrophilic co-solvents, such as ethylene glycol and dimethyl sulfoxide, were more tolerated. The effects of co-solvents at low concentration were different for each enzyme-solvent pair. The increase in DbjA activitywas induced by the majority of organic co-solvents tested, while activities of DhaA and LinB decreased at comparable concentrations of the same co-solvent. Moreover, high increase of DbjA enantioselectivity was observed.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biotechnology Journal

ISSN

1860-6768

e-ISSN

—

Volume of the periodical

8

Issue of the periodical within the volume

6

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

719-729

UT code for WoS article

000320031900013

EID of the result in the Scopus database

—