HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00065053" target="_blank" >RIV/00159816:_____/16:00065053 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/16:00088547 RIV/00216305:26230/16:PU122827

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gkw416" target="_blank" >http://dx.doi.org/10.1093/nar/gkw416</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkw416" target="_blank" >10.1093/nar/gkw416</a>

Result language

angličtina

Original language name

HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering

Original language description

Nucleic Acids Research, 2016 1 doi: 10.1093/nar/gkw416 HotSpot Wizard 2.0: automated design of site-specific mutations and smart libraries in protein engineering Jaroslav Bendl 1,2,3, + , Jan Stourac 1, + , Eva Sebestova 1 , Ondrej Vavra 1 , Milos Musil 1,2 , Jan Brezovsky 1,3,* and Jiri Damborsky 1,3,* 1 Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Masaryk University, 625 00 Brno, Czech Republic, 2 Department of Information Systems, Faculty of Information Technology, Brno University of Technology, 612 66 Brno, Czech Republic and 3 International Clinical Research Center, St. Anne's University Hospital Brno, Brno, Czech Republic Received February 21, 2016; Revised April 22, 2016; Accepted May 03, 2016 ABSTRACT HotSpot Wizard 2.0 is a web server for automated identification of hot spots and design of smart li- braries for engineering proteins' stability, catalytic activity, substrate specificity and enantioselectivity. The server integrates sequence, structural and evo- lutionary information obtained from 3 databases and 20 computational tools. Users are guided through the processes of selecting hot spots using four dif- ferent protein engineering strategies and optimizing the resulting library's size by narrowing down a set of substitutions at individual randomized positions. The only required input is a query protein structure. The results of the calculations are mapped onto the protein's structure and visualized with a JSmol ap- plet. HotSpot Wizard lists annotated residues suit- able for mutagenesis and can automatically design appropriate codons for each implemented strategy. Overall, HotSpot Wizard provides comprehensive an- notations of protein structures and assists protein engineers with the rational design of site-specific mutations and focused libraries.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

44

Issue of the periodical within the volume

W1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

"W479"-"W487"

UT code for WoS article

000379786800079

EID of the result in the Scopus database

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