Maternal obesity alters endoplasmic reticulum homeostasis in offspring pancreas

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00065210" target="_blank" >RIV/00159816:_____/16:00065210 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s13105-016-0476-6" target="_blank" >http://dx.doi.org/10.1007/s13105-016-0476-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13105-016-0476-6" target="_blank" >10.1007/s13105-016-0476-6</a>

Result language

angličtina

Original language name

Maternal obesity alters endoplasmic reticulum homeostasis in offspring pancreas

Original language description

The prevalence of non-alcoholic fatty pancreas disease (NAFPD) is increasing in parallel with obesity rates. Stress-related alterations in endoplasmic reticulum (ER), such as the unfolded protein response (UPR), are associated with obesity. The aim of this study was to investigate ER imbalance in the pancreas of a mice model of adult and perinatal diet-induced obesity. Twenty female C57BL/6J mice were assigned to control (Con) or obesogenic (Ob) diets prior to and during pregnancy and lactation. Their offspring were weaned onto Con or Ob diets up to 6 months post-partum. Then, after sacrifice, plasma biochemical analyses, gene expression, and protein concentrations were measured in pancreata. Offspring of Ob-fed mice had significantly increased body weight (p < 0.001) and plasma leptin (p < 0.001) and decreased insulin (p < 0.01) levels. Maternal obesogenic diet decreased the total and phosphorylated Eif2 alpha and increased spliced X-box binding protein 1 (XBP1). Pancreatic gene expression of downstream regulators of UPR (EDEM, homocysteine-responsive endoplasmic reticulum-resident (HERP), activating transcription factor 4 (ATF4), and C/EBP homologous protein (CHOP)) and autophagy-related proteins (LC3BI/LC3BII) were differently disrupted by obesogenic feeding in both mothers and offspring (from p < 0.1 to p < 0.001). Maternal obesity and Ob feeding in their offspring alter UPR in NAFPD, with involvement of proapoptotic and autophagy-related markers. Upstream and downstream regulators of PERK, IRE1 alpha, and ATF6 pathways were affected differently following the obesogenic insults.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Physiology and Biochemistry

ISSN

1138-7548

e-ISSN

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Volume of the periodical

72

Issue of the periodical within the volume

2

Country of publishing house

ES - SPAIN

Number of pages

11

Pages from-to

281-291

UT code for WoS article

000376479400013

EID of the result in the Scopus database

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