Maternal obesity alters endoplasmic reticulum homeostasis in offspring pancreas
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00065210" target="_blank" >RIV/00159816:_____/16:00065210 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s13105-016-0476-6" target="_blank" >http://dx.doi.org/10.1007/s13105-016-0476-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s13105-016-0476-6" target="_blank" >10.1007/s13105-016-0476-6</a>
Alternative languages
Result language
angličtina
Original language name
Maternal obesity alters endoplasmic reticulum homeostasis in offspring pancreas
Original language description
The prevalence of non-alcoholic fatty pancreas disease (NAFPD) is increasing in parallel with obesity rates. Stress-related alterations in endoplasmic reticulum (ER), such as the unfolded protein response (UPR), are associated with obesity. The aim of this study was to investigate ER imbalance in the pancreas of a mice model of adult and perinatal diet-induced obesity. Twenty female C57BL/6J mice were assigned to control (Con) or obesogenic (Ob) diets prior to and during pregnancy and lactation. Their offspring were weaned onto Con or Ob diets up to 6 months post-partum. Then, after sacrifice, plasma biochemical analyses, gene expression, and protein concentrations were measured in pancreata. Offspring of Ob-fed mice had significantly increased body weight (p < 0.001) and plasma leptin (p < 0.001) and decreased insulin (p < 0.01) levels. Maternal obesogenic diet decreased the total and phosphorylated Eif2 alpha and increased spliced X-box binding protein 1 (XBP1). Pancreatic gene expression of downstream regulators of UPR (EDEM, homocysteine-responsive endoplasmic reticulum-resident (HERP), activating transcription factor 4 (ATF4), and C/EBP homologous protein (CHOP)) and autophagy-related proteins (LC3BI/LC3BII) were differently disrupted by obesogenic feeding in both mothers and offspring (from p < 0.1 to p < 0.001). Maternal obesity and Ob feeding in their offspring alter UPR in NAFPD, with involvement of proapoptotic and autophagy-related markers. Upstream and downstream regulators of PERK, IRE1 alpha, and ATF6 pathways were affected differently following the obesogenic insults.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Physiology and Biochemistry
ISSN
1138-7548
e-ISSN
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Volume of the periodical
72
Issue of the periodical within the volume
2
Country of publishing house
ES - SPAIN
Number of pages
11
Pages from-to
281-291
UT code for WoS article
000376479400013
EID of the result in the Scopus database
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