Stress of endoplasmic reticulum modulates differentiation and lipogenesis of human adipocytes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909573" target="_blank" >RIV/00216208:11120/15:43909573 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.bbrc.2015.03.090" target="_blank" >http://dx.doi.org/10.1016/j.bbrc.2015.03.090</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bbrc.2015.03.090" target="_blank" >10.1016/j.bbrc.2015.03.090</a>

Result language
angličtina
Original language name
Stress of endoplasmic reticulum modulates differentiation and lipogenesis of human adipocytes
Original language description
Background: Adipocytes are cells specialized for storage of neutral lipids. This storage capacity is dependent on lipogenesis and is diminished in obesity. The reason for the decline in lipogenic activity of adipocytes in obesity remains unknown. Recentdata show that lipogenesis in liver is regulated by pathways initiated by endoplasmic reticulum stress (ERS). Thus, we aimed at investigating the effect of ERS on lipogenesis in adipose cells. Methods: Preadipocytes were isolated from subcutaneous abdominal adipose tissue from obese volunteers and in vitro differentiated into adipocytes. ERS was induced pharmacologically by thapsigargin (TG) or tunicamycin (TM). Activation of Unfolded Protein Response pathway (UPR) was monitored on the level of eIF2a phosphorylation and mRNA expression of downstream targets of UPR sensors. Adipogenic and lipogenic capacity was evaluated by Oil Red O staining, measurement of incorporation of radio-labelled glucose or acetic acid into lipids and mRNA anal
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GAP301%2F11%2F0748" target="_blank" >GAP301/11/0748: The role of endoplasmic reticulum stress in the pathogenesis of obesity-related inflammation in human adipose tissue</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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