Melatonin promotes cardiomyogenesis of embryonic stem cells via inhibition of HIF-1α stabilization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00066010" target="_blank" >RIV/00159816:_____/16:00066010 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/jpi.12366" target="_blank" >http://dx.doi.org/10.1111/jpi.12366</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jpi.12366" target="_blank" >10.1111/jpi.12366</a>

Result language

angličtina

Original language name

Melatonin promotes cardiomyogenesis of embryonic stem cells via inhibition of HIF-1α stabilization

Original language description

Melatonin, a molecule involved in the regulation of circadian rhythms, has protective effects against myocardial injuries. However, its capability to regulate the maturation of cardiac progenitor cells is unclear. Recently, several studies have shown that me- latonin inhibits the stabilization of hypoxia- inducible factors (HIFs), important sign- aling molecules with cardioprotective effects. In this study, by employing differentiating mouse embryonic stem cells, we report that melatonin significantly upregulated the expression of cardiac cell- specific markers (myosin heavy chains six and seven) as well as the percentage of myosin heavy chain- positive cells. Importantly, melatonin decreased HIF- 1α stabilization and transcriptional activity and, in con- trast, induced HIF- 2α stabilization. Interestingly, the deletion of HIF- 1α completely inhibited the pro- cardiomyogenic effect of melatonin as well as the melatonin- mediated HIF- 2α stabilization. Moreover, melatonin increased Sirt- 1 levels in a HIF- 1α- dependent manner. Taken together, we provide new evidence of a time- specific inhibition of HIF- 1α stabilization as an essential feature of melatonin- induced car- diomyogenesis and unexpected different roles of HIF- 1α stabilization during various stages of cardiac development. These results uncover new mechanisms underlying the maturation of cardiac progenitor cells and can help in the development of novel strategies for using melatonin in cardiac regeneration therapy.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FH - Neurology, neuro-surgery, nuero-sciences

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Pineal Research

ISSN

0742-3098

e-ISSN

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Volume of the periodical

61

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

493-503

UT code for WoS article

000386357100007

EID of the result in the Scopus database

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