One reporter for in-cell activity profiling of majority of protein kinase oncogenes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00066925" target="_blank" >RIV/00159816:_____/17:00066925 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/17:00094746 RIV/65269705:_____/17:00066925

Result on the web

<a href="http://dx.doi.org/10.7554/eLife.21536" target="_blank" >http://dx.doi.org/10.7554/eLife.21536</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.7554/eLife.21536" target="_blank" >10.7554/eLife.21536</a>

Result language

angličtina

Original language name

One reporter for in-cell activity profiling of majority of protein kinase oncogenes

Original language description

In-cell profiling enables the evaluation of receptor tyrosine activity in a complex environment of regulatory networks that affect signal initiation, propagation and feedback. We used FGF-receptor signaling to identify EGR1 as a locus that strongly responds to the activation of a majority of the recognized protein kinase oncogenes, including 30 receptor tyrosine kinases and 154 of their disease-associated mutants. The EGR1 promoter was engineered to enhance trans-activation capacity and optimized for simple screening assays with luciferase or fluorescent reporters. The efficacy of the developed, fully synthetic reporters was demonstrated by the identification of novel targets for two clinically used tyrosine kinase inhibitors, nilotinib and osimertinib. A universal reporter system for in-cell protein kinase profiling will facilitate repurposing of existing anti-cancer drugs and identification of novel inhibitors in high-throughput screening studies.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30101 - Human genetics

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

eLife

ISSN

2050-084X

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

FEB

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

"e21536"

UT code for WoS article

000394260500001

EID of the result in the Scopus database

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