Comparison of various methods of ischaemic cardioprotection on vitality of rat heart grafts

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00067049" target="_blank" >RIV/00159816:_____/17:00067049 - isvavai.cz</a>

Alternative codes found

RIV/62157124:16170/17:43875748 RIV/62157124:16370/17:43875748

Result on the web

<a href="http://dx.doi.org/10.2754/avb201786020199" target="_blank" >http://dx.doi.org/10.2754/avb201786020199</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201786020199" target="_blank" >10.2754/avb201786020199</a>

Result language

angličtina

Original language name

Comparison of various methods of ischaemic cardioprotection on vitality of rat heart grafts

Original language description

The aim of the study was to compare 4 modes of ischaemic cardioprotection using continuous prograde autologous blood perfusion of the coronary artery in two hypothermic modes (group A, B) or conventional protection by cooled Hartmann solution (group C) or cooled saline (group D) without perfusion of the graft. Male Wistar rats (n = 24) were divided into four groups (A-D). In groups A (22-25 oC) and B (4-8 oC), blood perfusion rate was 10 ml/h and the graft was placed in a water bath. Groups C, D were initially rinsed with cold (4-8 oC) Hartmann solution (C) and cold saline solution (D), next the graft was placed in a water bath of cold (4-8 oC) Hartmann solution (C) or saline solution (D). The observed time was 30 min after the implemented perfusion (A, B) or initial rinsing (C, D). At 30 min, hearts of all the groups were perfused for 10 min with prograde-autologous arterialized blood at room temperature. At perfusion minute 10, blood was collected for biochemical analysis (sample 1). Sample 2 involved blood from a portable syringe infusion pumps (in parallel with sample 1). Pairwise test differences between samples 1 and 2 were signifcant in all the groups as regards creatine kinase and lactate dehydrogenase values, sampling 1 values being always higher, while cardiac troponin I concentrations were nonsignifcant in the same comparison. The heart rate during the fnal perfusion was identical in all the groups. Our study has demonstrated that all observed cardioprotection modes are useful for experimental heart grafting

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40301 - Veterinary science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

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Volume of the periodical

86

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

199-206

UT code for WoS article

000405584800012

EID of the result in the Scopus database

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