Moderate hypothermia during ex vivo machine perfusion promotes recovery of hearts donated after cardiocirculatory death
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F16%3A00089079" target="_blank" >RIV/00216224:14110/16:00089079 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1093/ejcts/ezv066" target="_blank" >http://dx.doi.org/10.1093/ejcts/ezv066</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/ejcts/ezv066" target="_blank" >10.1093/ejcts/ezv066</a>
Alternative languages
Result language
angličtina
Original language name
Moderate hypothermia during ex vivo machine perfusion promotes recovery of hearts donated after cardiocirculatory death
Original language description
OBJECTIVES To establish the optimal machine perfusion temperature for recovery of hearts in a rodent model of donation after declaration of cardiocirculatory death (DCD). METHODS Hearts from male Lewis rats (n = 14/group) were subjected to 25 min of in situ warm (37°C) ischaemia to simulate DCD. They were then explanted and reperfused with diluted autologous blood for 60 min at 20, 25, 30, 33 or 37°C, after which they were stored at 0-4°C in Custodiol preservation solution for 240 min. Fresh-excised and cold-stored ischaemic hearts were used as controls. The viability of the different groups was assessed by comparing heart rate and left ventricular contractility in a Langendorff circuit, as well as perfusate levels of troponin-t and creatine kinase (CK), and myocardial levels of adenosine triphosphate (ATP) and reduced glutathione. RESULTS During ex vivo reperfusion, hearts in all groups resumed beating within minutes. The mean heart rate was highest in the 37°C group at 154.72 +/- 33.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Journal Cardio-Thoracic Surgery
ISSN
1010-7940
e-ISSN
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Volume of the periodical
49
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
25-31
UT code for WoS article
000370979800012
EID of the result in the Scopus database
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