Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F05%3APU50087" target="_blank" >RIV/00216305:26220/05:PU50087 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models

Original language description

Voltage-sensitive dyes (VSD's) can be used in touch-less recording of action potentials from animal hearts. VSD's undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field. This optical response is sufficiently fast to detect transient potential changes in excitable cardiac cells. Two animal models are employed in our laboratory to record monophasic action potentials using VSD in isolated hearts perfused accorrding to Langendorf - guinea pig and rabbit. The hearts of both species undergo the same isolation and loading procedures. Briefly, the spontaneously beating heart is mounted on Langendorf set and perfused with constant perfusion pressure with Krebs-Henseleit solution (37°C, 1,25mM Ca2+). After control period (20min), the heart is loaded with di-4-ANEPPS (2uM) diluted in perfusion solution (25 min) and then washed-out with dye-free perfusion solution (25 min). It has been observed that durin

Czech name

Comparison of the tissue response during the loading with voltage-sensitive dye in two animal models

Czech description

Voltage-sensitive dyes (VSD's) can be used in touch-less recording of action potentials from animal hearts. VSD's undergo changes in their electronic structure, and consequently their fluorescence spectra, in response to changes in the surrounding electric field. This optical response is sufficiently fast to detect transient potential changes in excitable cardiac cells. Two animal models are employed in our laboratory to record monophasic action potentials using VSD in isolated hearts perfused accorrding to Langendorf - guinea pig and rabbit. The hearts of both species undergo the same isolation and loading procedures. Briefly, the spontaneously beating heart is mounted on Langendorf set and perfused with constant perfusion pressure with Krebs-Henseleit solution (37°C, 1,25mM Ca2+). After control period (20min), the heart is loaded with di-4-ANEPPS (2uM) diluted in perfusion solution (25 min) and then washed-out with dye-free perfusion solution (25 min). It has been observed that durin

Type

D - Article in proceedings

CEP classification

JD - Use of computers, robotics and its application

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Computers in Cardiology

ISBN

0-7803-8894-1

ISSN

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e-ISSN

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Number of pages

4

Pages from-to

1-4

Publisher name

IEEE

Place of publication

Francie

Event location

Lyon

Event date

Sep 25, 2005

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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