FireProt: web server for automated design of thermostable proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00067126" target="_blank" >RIV/00159816:_____/17:00067126 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/17:00095410 RIV/00216305:26230/17:PU126411

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gkx285" target="_blank" >http://dx.doi.org/10.1093/nar/gkx285</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkx285" target="_blank" >10.1093/nar/gkx285</a>

Result language

angličtina

Original language name

FireProt: web server for automated design of thermostable proteins

Original language description

There is a continuous interest in increasing proteins stability to enhance their usability in numerous biomedical and biotechnological applications. A number of in silico tools for the prediction of the effect of mutations on protein stability have been developed recently. However, only single-point mutations with a small effect on protein stability are typically predicted with the existing tools and have to be followed by laborious protein expression, purification, and characterization. Here, we present FireProt, a web server for the automated design of multiple-point thermostable mutant proteins that combines structural and evolutionary information in its calculation core. FireProt utilizes sixteen tools and three protein engineering strategies for making reliable protein designs. The server is complemented with interactive, easy-to-use interface that allows users to directly analyze and optionally modify designed thermostable mutants. FireProt is freely available at http://loschmidt.chemi.muni.cz/fireprot.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

—

Volume of the periodical

45

Issue of the periodical within the volume

W1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

7

Pages from-to

"W393"-"W399"

UT code for WoS article

000404427000059

EID of the result in the Scopus database

—