FireProt 2.0: web-based platform for the fully automated design of thermostable proteins

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F23%3A00079772" target="_blank" >RIV/00159816:_____/23:00079772 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26230/23:PU149790

Result on the web

<a href="https://academic.oup.com/bib/article/25/1/bbad425/7453438" target="_blank" >https://academic.oup.com/bib/article/25/1/bbad425/7453438</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/bib/bbad425" target="_blank" >10.1093/bib/bbad425</a>

Result language

angličtina

Original language name

FireProt 2.0: web-based platform for the fully automated design of thermostable proteins

Original language description

Thermostable proteins find their use in numerous biomedical and biotechnological applications. However, the computational design of stable proteins often results in single-point mutations with a limited effect on protein stability. However, the construction of stable multiple-point mutants can prove difficult due to the possibility of antagonistic effects between individual mutations. FireProt protocol enables the automated computational design of highly stable multiple-point mutants. FireProt 2.0 builds on top of the previously published FireProt web, retaining the original functionality and expanding it with several new stabilization strategies. FireProt 2.0 integrates the AlphaFold database and the homology modeling for structure prediction, enabling calculations starting from a sequence. Multiple-point designs are constructed using the Bron-Kerbosch algorithm minimizing the antagonistic effect between the individual mutations. Users can newly limit the FireProt calculation to a set of user-defined mutations, run a saturation mutagenesis of the whole protein or select rigidifying mutations based on B-factors. Evolution-based back-to-consensus strategy is complemented by ancestral sequence reconstruction. FireProt 2.0 is significantly faster and a reworked graphical user interface broadens the tool&apos;s availability even to users with older hardware. FireProt 2.0 is freely available at http://loschmidt.chemi.muni.cz/fireprotweb.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10609 - Biochemical research methods

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

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Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Briefings in Bioinformatics

ISSN

1467-5463

e-ISSN

1477-4054

Volume of the periodical

25

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

"bbad425"

UT code for WoS article

001173375300007

EID of the result in the Scopus database

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