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The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26230%2F23%3APR37705" target="_blank" >RIV/00216305:26230/23:PR37705 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.fit.vut.cz/research/product/792/" target="_blank" >https://www.fit.vut.cz/research/product/792/</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    FireProt 2.0

  • Original language description

    FireProt protocol enables the automated computational design of highly stable multiple-point mutants. FireProt 2.0 builds on top of the previously published FireProt web, retaining the original functionality and expanding it with several new stabilization strategies. FireProt 2.0 integrates the AlphaFold database and the homology modelling for structure prediction, enabling calculations starting from a sequence. Multiple-point designs are constructed using the Bron-Kerbosch algorithm minimizing the antagonistic effect between the individual mutations. Users can newly limit the FireProt calculation to a set of user-defined mutations, run a saturation mutagenesis of the whole protein, or select rigidifying mutations based on B-factors. Evolution-based back-to-consensus strategy is complemented by ancestral sequence reconstruction. FireProt 2.0 is significantly faster and a reworked graphical user interface broadens the tool's availability even to users with older hardware.

  • Czech name

  • Czech description

Classification

  • Type

    R - Software

  • CEP classification

  • OECD FORD branch

    10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Internal product ID

    FireProt

  • Technical parameters

    Pro informace o licenčních podmínkách prosím kontaktujte: Mgr. Radka Báčová, Výzkumné centrum informačních technologií, Fakulta informačních technologií VUT v Brně, Božetěchova 2, 612 66 Brno, 541 141 473.

  • Economical parameters

    Nástroj je volně dostupný pro akademickou komunitu. Nelze použít pro komerční účely (z důvodu ochrany práv třetích stran - několika integrovaných metod s nesvobodnou licencí).

  • Owner IČO

    00216224

  • Owner name

    Masarykova Univerzita v Brně