Role of PCNA and RFC in promoting Mus81-complex activity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00067613" target="_blank" >RIV/00159816:_____/17:00067613 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/17:00095209
Result on the web
<a href="http://dx.doi.org/10.1186/s12915-017-0429-8" target="_blank" >http://dx.doi.org/10.1186/s12915-017-0429-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12915-017-0429-8" target="_blank" >10.1186/s12915-017-0429-8</a>
Alternative languages
Result language
angličtina
Original language name
Role of PCNA and RFC in promoting Mus81-complex activity
Original language description
Background: Proper DNA replication is essential for faithful transmission of the genome. However, replication stress has serious impact on the integrity of the cell, leading to stalling or collapse of replication forks, and has been determined as a driving force of carcinogenesis. Mus81-Mms4 complex is a structure-specific endonuclease previously shown to be involved in processing of aberrant replication intermediates and promotes POLD3-dependent DNA synthesis via break-induced replication. However, how replication components might be involved in this process is not known. Results: Herein, we show the interaction and robust stimulation of Mus81-Mms4 nuclease activity by heteropentameric replication factor C(RFC) complex, the processivity factor of replicative DNA polymerases that is responsible for loading of proliferating cell nuclear antigen (PCNA) during DNA replication and repair. This stimulation is enhanced by RFC-dependent ATP hydrolysis and by PCNA loading on the DNA. Moreover, this stimulation is not specific to Rfc1, the largest of subunit of this complex, thus indicating that alternative clamp loaders may also play a role in the stimulation. We also observed a targeting of Mus81 by RFC to the nick-containing DNA substrate and we provide further evidence that indicates cooperation between Mus81 and the RFC complex in the repair of DNA lesions generated by various DNA-damaging agents. Conclusions: Identification of new interacting partners and modulators of Mus81-Mms4 nuclease, RFC, and PCNA imply the cooperation of these factors in resolution of stalled replication forks and branched DNA structures emanating from the restarted replication forks under conditions of replication stress.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30101 - Human genetics
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC Biology
ISSN
1741-7007
e-ISSN
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Volume of the periodical
15
Issue of the periodical within the volume
OCT
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
90
UT code for WoS article
000412075900002
EID of the result in the Scopus database
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