Serological diagnostics in the detection of IgG autoantibodies against human collagen VII in epidermolysis bullosa acquisita: a multicentre analysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00068312" target="_blank" >RIV/00159816:_____/17:00068312 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/17:00099150

Result on the web

<a href="http://dx.doi.org/10.1111/bjd.15800" target="_blank" >http://dx.doi.org/10.1111/bjd.15800</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/bjd.15800" target="_blank" >10.1111/bjd.15800</a>

Result language

angličtina

Original language name

Serological diagnostics in the detection of IgG autoantibodies against human collagen VII in epidermolysis bullosa acquisita: a multicentre analysis

Original language description

BackgroundEpidermolysis bullosa acquisita (EBA) is a rare, potentially devastating autoimmune disease of the skin. IgG autoantibodies directed against type VII collagen (Col7), the major component of anchoring fibrils, induce skin fragility leading to cutaneous and mucocutaneous blister formation, which is mostly of a scarring phenotype. Thus, powerful and reproducible diagnostic assays are critical to establish the diagnosis of EBA early to avoid irreversible sequelae. ObjectivesThe present international, retrospective multicentre study included a large cohort of patients with EBA and evaluated the diagnostic power of four different diagnostic assays for the detection of anti-Col7 IgG autoantibodies. MethodsOverall, 95 EBA sera and 200 control sera consisting of 100 bullous pemphigoid sera, 50 pemphigus vulgaris sera and 50 sera of healthy controls were tested for anti-Col7 IgG autoantibodies using indirect immunofluorescence (IIF), two commercial enzyme-linked immunosorbent assay (ELISA) systems and Western blot (WB) analysis. EBA sera were taken from patients with positive direct immunofluorescence and IgG reactivity in at least one of the immunoserological assays (IIF, ELISA, WB). ResultsA Col7-NC1/NC2 ELISA (MBL, Nagoya, Japan) showed the highest sensitivity (979%), followed by a Col7-NC1 ELISA (Euroimmun, Lubeck, Germany) (895%), WB with Col7-NC1 (853%), and IIF on saline-split human skin (747%). The specificities of both ELISA systems were comparable (NC1 987%, NC1/NC2 993%). Furthermore, WB was more sensitive than IIF, which was more specific. ConclusionsThe two commercially available ELISA systems allow for a highly sensitive and specific diagnosis of EBA. The sensitivity of the Col7-NC1/NC2 ELISA is significantly higher compared with the ELISA based on the Col7-NC1 domain only. What&apos;s already known about this topic? ...

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30216 - Dermatology and venereal diseases

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

British Journal of Dermatology

ISSN

0007-0963

e-ISSN

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Volume of the periodical

177

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

1683-1692

UT code for WoS article

000419479300057

EID of the result in the Scopus database

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