Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F18%3A00068657" target="_blank" >RIV/00159816:_____/18:00068657 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/18:00492427 RIV/00216224:14310/18:00106565 RIV/00209805:_____/18:00078101
Result on the web
<a href="http://dx.doi.org/10.1038/bjc.2017.497" target="_blank" >http://dx.doi.org/10.1038/bjc.2017.497</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/bjc.2017.497" target="_blank" >10.1038/bjc.2017.497</a>

Result language
angličtina
Original language name
Plasticity and intratumoural heterogeneity of cell surface antigen expression in breast cancer
Original language description
Background: The intratumoural heterogeneity, often driven by epithelial-to-mesenchymal transition (EMT), significantly contributes to chemoresistance and disease progression in adenocarcinomas. Methods: We introduced a high-throughput screening platform to identify surface antigens that associate with epithelial-mesenchymal plasticity in well-defined pairs of epithelial cell lines and their mesenchymal counterparts. Using multicolour flow cytometry, we then analysed the expression of 10 most robustly changed antigens and identified a 10-molecule surface signature, in pan-cytokeratin-positive/EpCAM-positive and -negative fractions of dissociated breast tumours. Results: We found that surface CD9, CD29, CD49c, and integrin beta 5 are lost in breast cancer cells that underwent EMT in vivo. The tetraspanin family member CD9 was concordantly downregulated both in vitro and in vivo and associated with epithelial phenotype and favourable prognosis. Conclusions: We propose that overall landscape of 10-molecule surface signature expression reflects the epithelial-mesenchymal plasticity in breast cancer.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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