Evaluation of miRNA detection methods for the analytical characteristics necessary for clinical utilization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F19%3A00071061" target="_blank" >RIV/00159816:_____/19:00071061 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/19:00111634 RIV/00209805:_____/19:00078353
Result on the web
<a href="https://www.future-science.com/doi/pdf/10.2144/btn-2019-0021" target="_blank" >https://www.future-science.com/doi/pdf/10.2144/btn-2019-0021</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2144/btn-2019-0021" target="_blank" >10.2144/btn-2019-0021</a>
Alternative languages
Result language
angličtina
Original language name
Evaluation of miRNA detection methods for the analytical characteristics necessary for clinical utilization
Original language description
miRNAs are promising biomarkers but methods for their measurement are not clear. We therefore examined three miRNA detection technologies and considered the analytical characteristics essential for clinical utilization. TaqMan assays, SplintR-qPCR and miREIA were compared for their absolute quantification bias, conformity and robustness. Absolute concentrations of miR-142-5p, miR-23a-3p and miR-93-5p were measured with all three methods using 30 samples. Robustness was evaluated by measurement of miR-21-5p in five uniform experiments. Correlations were miRNA-specific, but we observed a different absolute concentration range in RT-qPCR (fmol/mu l) and methods evading the RT process (amol/mu l). Consistently, RT-less methods reported better robustness (CV 8-19%) than RT-qPCR (CV 39-50%). The calibration curve in TaqMan Advanced assay was influenced by dilution media. Methods avoiding RT seem to be a promising future alternative for miRNA measurement. METHOD SUMMARY Three miRNA detection technologies were compared: 1) RT-qPCR where the RT step was performed with either a specific (TaqMan miRNA assay) or universal (TaqMan Advanced assay) priming strategy; 2) miREIA technology, using hybridization and specific antibody to DNA/RNA hybrids and 3) SplintR-qPCR, which utilizes a hybridization and ligation step followed by qPCR.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BIOTECHNIQUES
ISSN
0736-6205
e-ISSN
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Volume of the periodical
66
Issue of the periodical within the volume
6
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
277-284
UT code for WoS article
000474765200006
EID of the result in the Scopus database
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