Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F19%3A00072475" target="_blank" >RIV/00159816:_____/19:00072475 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14330/19:00110913

Result on the web

<a href="https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/quantitative-assessment-of-anticancer-drug-efficacy-from-coregistered-mass-spectrometry-and-fluorescence-microscopy-images-of-multicellular-tumor-spheroids/BA424253309B3ED823925DF7FDAA7D8C#" target="_blank" >https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/quantitative-assessment-of-anticancer-drug-efficacy-from-coregistered-mass-spectrometry-and-fluorescence-microscopy-images-of-multicellular-tumor-spheroids/BA424253309B3ED823925DF7FDAA7D8C#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S1431927619014983" target="_blank" >10.1017/S1431927619014983</a>

Result language

angličtina

Original language name

Quantitative Assessment of Anti-Cancer Drug Efficacy From Coregistered Mass Spectrometry and Fluorescence Microscopy Images of Multicellular Tumor Spheroids

Original language description

Spheroids-three-dimensional aggregates of cells grown from a cancer cell line-represent a model of living tissue for chemotherapy investigation. Distribution of chemotherapeutics in spheroid sections was determined using the matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI). Proliferating or apoptotic cells were immunohistochemically labeled and visualized by laser scanning confocal fluorescence microscopy (LSCM). Drug efficacy was evaluated by comparing coregistered MALDI MSI and LSCM data of drug-treated spheroids with LSCM only data of untreated control spheroids. We developed a fiducial-based workflow for coregistration of low-resolution MALDI MS with high-resolution LSCM images. To allow comparison of drug and cell distribution between the drug-treated and untreated spheroids of different shapes or diameters, we introduced a common diffusion-related coordinate, the distance from the spheroid boundary. In a procedure referred to as &quot;peeling&quot;, we correlated average drug distribution at a certain distance with the average reduction in the affected cells between the untreated and the treated spheroids. This novel approach makes it possible to differentiate between peripheral cells that died due to therapy and the innermost cells which died naturally. Two novel algorithms-for MALDI MS image denoising and for weighting of MALDI MSI and LSCM data by the presence of cell nuclei-are also presented.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20501 - Materials engineering

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microscopy and Microanalysis

ISSN

1431-9276

e-ISSN

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Volume of the periodical

25

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

1311-1322

UT code for WoS article

000502313700003

EID of the result in the Scopus database

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