Cell based AFM biosensensing for screening of pulmonary-drug related arrhytmic effects

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F21%3A00074466" target="_blank" >RIV/00159816:_____/21:00074466 - isvavai.cz</a>

Alternative codes found

RIV/65269705:_____/21:00074466 RIV/00216224:14110/21:00120083

Result on the web

<a href="http://dx.doi.org/10.37904/nanocon.2020.3745" target="_blank" >http://dx.doi.org/10.37904/nanocon.2020.3745</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.37904/nanocon.2020.3745" target="_blank" >10.37904/nanocon.2020.3745</a>

Result language

angličtina

Original language name

Cell based AFM biosensensing for screening of pulmonary-drug related arrhytmic effects

Original language description

Atomic force microscopy (AFM) combined with stem cell derived human cardiomyocytes (CM) enables dynamic follow-up of cardiac contractions (e.g. beating rate, contraction and relaxation times), simultaneously with other CM biomechanical properties. Today, majority of drugs entering clinical usage needs to be tested for adverse arrhythmic effects; nevertheless, the effects on cardiomyocyte contraction are not routinely employed, only when related to cardiac pathologies. AFM-based biosensor allows in-vitro disease modeling, but also enables to monitor the effect of CM-contraction affecting drugs. Until today only few selected drugs modulating contractility and spontaneous pacing were described in animal models. This work for the first time demonstrates that basic biomechanical parameters, such as average value of contraction force and the beat rate, represent valuable pharmacological indicators of different phenotypic effects on cells without genetic burden. The presented method is robust and has low computational requirements, while keeping optimal spatial sensitivity (force detection limit 200 pN, corresponding to 20 nm displacement). The cardiac stimulating activities of drugs utilized in pneumology as aminophylline, ipratropium, and salbutamol were tested. Stimulating drugs, e.g. methylxanthines and caffeine, presented aberrant cardiomyocyte response, confirming arrhythmogenic potential, and force related fluctuations. Quantification of spontaneous contraction irregularities and related contractility changes allow precise scaling of potential negative effects adding new safety level to clinically relevant drug testing. AFM combined with human CMs serve as robust real-time screening platform for effects of pulmonary drugs. Here we describe changes in CM contractility, which is hard to describe by other screening methods and was never tested with described medication.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings 12th International Conference on Nanomaterials - Research &amp; Application

ISBN

978-80-87294-98-7

ISSN

—

e-ISSN

—

Number of pages

6

Pages from-to

404-409

Publisher name

TANGER LTD

Place of publication

Ostrava

Event location

Brno

Event date

Oct 21, 2020

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000664505500069