Effects of salbutamol and ipratropium on human stem-cells derived cardiomyocytes’ function
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F22%3A00129720" target="_blank" >RIV/00216224:14110/22:00129720 - isvavai.cz</a>
Result on the web
<a href="https://erj.ersjournals.com/content/60/suppl_66/560" target="_blank" >https://erj.ersjournals.com/content/60/suppl_66/560</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1183/13993003.congress-2022.560" target="_blank" >10.1183/13993003.congress-2022.560</a>
Alternative languages
Result language
angličtina
Original language name
Effects of salbutamol and ipratropium on human stem-cells derived cardiomyocytes’ function
Original language description
Introduction: Patients with obstructive pulmonary diseases are treated by beta-2-agonists, parasympatholytics, and methylxanthines for long periods. Chronic use of these drugs may result in impairment of the cardiovascular system. Direct effects of these bronchodilators on the function of human cardiomyocytes are described insufficiently. Aims: We aimed to investigate in vitro effects of salbutamol and ipratropium on human-induced pluripotent stem cell-derived cardiomyocyte (hiPS-CMs) cell clusters. We focused on their chronotropic and inotropic effects. Methods: The measurements of 3D clusters of hiPS-CMs, embryoid bodies (EBs), with spontaneous pacing and contraction abilities were performed using atomic force microscopy (AFM). AFM enables real-time monitoring of EBs’ beating frequency and is capable of measuring its contractility. We measured the effect of increasing concentrations of salbutamol and ipratropium on EBs. Results: We treated 25 EBs with salbutamol and compared results with the control group of 11 EBs. Similarly, we tested 11 EBs with ipratropium. We observed significant positive chronotropic and inotropic effects in higher concentrations of salbutamol with statistically significant elevation of contraction force for clinically relevant salbutamol concentration. Measurements with ipratropium did not reveal any changes in EBs’ electromechanical function. Conclusions: The effect of pulmonary drugs on the cardiovascular system can be reliably tested using a combination of AFM and hiPS-CMs. The clinically relevant concentration of salbutamol causes a significant elevation of human cardiomyocytes’ contractility and beating frequency.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
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OECD FORD branch
30203 - Respiratory systems
Result continuities
Project
<a href="/en/project/NU20-06-00156" target="_blank" >NU20-06-00156: The effect of bronchodilator drugs on human cardiomyocytes.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů