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Human Cytomegalovirus and Epstein-Barr virus specific immunity in patients with ulcerative colitis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F21%3A00074742" target="_blank" >RIV/00159816:_____/21:00074742 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14110/21:00122152

  • Result on the web

    <a href="https://link.springer.com/article/10.1007%2Fs10238-021-00702-2" target="_blank" >https://link.springer.com/article/10.1007%2Fs10238-021-00702-2</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s10238-021-00702-2" target="_blank" >10.1007/s10238-021-00702-2</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Human Cytomegalovirus and Epstein-Barr virus specific immunity in patients with ulcerative colitis

  • Original language description

    Human Cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) are endowed with the ability of establishing lifelong latency in human hosts and reactivating in immunocompromised subjects, including patients suffering from ulcerative colitis (UC). We, therefore, aimed to investigate virus-specific immunity in UC patients. A cohort of 24 UC patients (14 responders and 10 refractory to therapy) and 26 control subjects was prospectively enrolled to undergo virus-specific serology (by ELISA assay) and assessment of both CD4(+) and CD8(+) virus-specific T-cell response (by interferon-gamma enzyme-linked immunospotanalysis). In parallel, mucosal viral load was determined by quantitative real-time PCR and the values were correlated with both clinical and endoscopic indexes of activity. For statistics, the t-test, Mann-Withney test, Fisher&apos;s exact test and Spearman rank correlation test were applied; p &lt; 0.05 was considered significant. EBV-specific CD4(+) and CD8(+) T-cell responses were significantly lower in UC patients compared to controls (p &lt; 0.0001 and p = 0.0006, respectively), whereas no difference was found for HCMV-specific T-cell response. When dividing the UC group according to response to therapy, both responders and refractory UC patients showed a deficient EBV-specific CD4(+) T-cell response with respect to controls (p &lt; 0.04 and p = 0.0003, respectively). Moreover, both EBV and HCMV mucosal loads were significantly higher in refractory UC than in responders and controls (p = 0.007 and 0.003; and p = 0.02 and 0.001, respectively), and correlated with activity indexes. Steroid therapy seemed the main risk factor for triggering EBV colitis. Finally, no cases of IgM positivity were found in the study population. An impaired EBV-specific immunity was clearly evident in UC patients, mostly in those refractory to therapy. The ELISPOT assay may serve as new tool for quantifying and monitoring virus-specific T-cell immunity in UC.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30200 - Clinical medicine

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Clinical and Experimental Medicine

  • ISSN

    1591-8890

  • e-ISSN

  • Volume of the periodical

    21

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    10

  • Pages from-to

    379-388

  • UT code for WoS article

    000633271600001

  • EID of the result in the Scopus database