Immunological examination of peripheral blood in patients with colorectal cancer compared to healthy controls
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10385606" target="_blank" >RIV/00216208:11110/18:10385606 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1080/08820139.2018.1480030" target="_blank" >https://doi.org/10.1080/08820139.2018.1480030</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/08820139.2018.1480030" target="_blank" >10.1080/08820139.2018.1480030</a>
Alternative languages
Result language
angličtina
Original language name
Immunological examination of peripheral blood in patients with colorectal cancer compared to healthy controls
Original language description
Background: The objective of this study was to investigate serum levels of immunosuppressive cytokines TGF beta 1 and VEGF and count of immune cells in peripheral blood in stage II and III colorectal cancer patients.Methods: Blood samples were collected from 22 colorectal patients and 25 healthy controls before the start of treatment. All patients were examined by a clinical immunologist to exclude patients with immune disorders and autoimmune diseases. TGF beta 1 and VEGF were measured by ELISA, and anti-tumor cellular immunity cells (CD4, CD8, B cells, NK cells) were measured by flow cytometry.Results: TGF beta 1 and VEGF plasma levels were significantly increased in stage II and III colorectal patients compared with control group (both p<0.0001). A decrease in the cellular immunity was shown in the absolute numbers of cytotoxic T lymphocytes (CD8+ ; p=0.0240), helper T lymphocytes (CD4+ ; p=0.0019), and natural killer cells (CD16 + CD56+; p<0.0001) in both stage II and stage III patients. On the contrary, B lymphocyte (CD19+) serum levels were increased in colon cancer patients (p<0.0001) compared to the control group.Conclusions: Our results show peripheral blood levels of TGF beta and VEGF were significantly increased in colorectal patients and changes in cellular anticancer immunity in comparison to control group. These results will be compared with results from Immunoscore.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30204 - Oncology
Result continuities
Project
<a href="/en/project/NV15-28188A" target="_blank" >NV15-28188A: Monitoring Cancer Microenvironment and Immune response in Adjudicating the Prognosis and therapy response in Patients with solid tumors</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Immunological Investigations
ISSN
0882-0139
e-ISSN
—
Volume of the periodical
47
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
643-653
UT code for WoS article
000452041600001
EID of the result in the Scopus database
2-s2.0-85048804245