The Manufacture of Xeno‐ and Feeder‐Free Clinical‐Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F22%3A00076402" target="_blank" >RIV/00159816:_____/22:00076402 - isvavai.cz</a>
Alternative codes found
RIV/65269705:_____/22:00076402 RIV/00216224:14110/22:00126955
Result on the web
<a href="https://www.mdpi.com/1422-0067/23/20/12500" target="_blank" >https://www.mdpi.com/1422-0067/23/20/12500</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms232012500" target="_blank" >10.3390/ijms232012500</a>

Result language
angličtina
Original language name
The Manufacture of Xeno‐ and Feeder‐Free Clinical‐Grade Human Embryonic Stem Cell Lines: First Step for Cell Therapy
Original language description
Human embryonic stem cells (hESCs) are increasingly used in clinical trials as they can change the outcome of treatment for many human diseases. They are used as a starting material for further differentiation into specific cell types and to achieve the desirable result of the cell therapy; thus, the quality of hESCs has to be taken into account. Therefore, current good manufacturing prac- tice (cGMP) has to be implemented in the transport of embryos, derivation of inner cell mass to xeno-free, feeder-free and defined hESC culture, and cell freezing. The in-depth characterization of hESC lines focused on safety, pluripotency, differentiation potential and genetic background has to complement this process. In this paper, we show the derivation of three clinical-grade hESC lines, MUCG01, MUCG02, and MUCG03, following these criteria. We developed and validated the sys- tem for the manufacture of xeno-free and feeder-free clinical-grade hESC lines that present high- quality starting material suitable for cell therapy according to cGMP.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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