Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F23%3A00078359" target="_blank" >RIV/00159816:_____/23:00078359 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/23:00131861 RIV/65269705:_____/23:00078359
Result on the web
<a href="https://www.nature.com/articles/s41598-023-42236-5.pdf" target="_blank" >https://www.nature.com/articles/s41598-023-42236-5.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-023-42236-5" target="_blank" >10.1038/s41598-023-42236-5</a>
Alternative languages
Result language
angličtina
Original language name
Truncated vitronectin with E-cadherin enables the xeno-free derivation of human embryonic stem cells
Original language description
Human embryonic stem cells (hESCs) have unique abilities that enable their use in cell therapy, disease modeling, and drug development. Their derivation is usually performed using a feeder layer, which is undefined and can potentially cause a contamination by xeno components, therefore there is a tendency to replace feeders with xeno-free defined substrates in recent years. Three hESC lines were successfully derived on the vitronectin with a truncated N-terminus (VTN-N) in combination with E-cadherin in xeno-free conditions for the first time, and their undifferentiated state, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers were confirmed. These results support the conclusion that the VTN-N/E-cadherin is a suitable substrate for the xeno-free derivation of hESCs and can be used for the derivation of hESCs according to good manufacturing practices.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20600 - Medical engineering
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
13
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
15062
UT code for WoS article
001067966700037
EID of the result in the Scopus database
2-s2.0-85170704358