Geometric Control of Cell Behavior by Biomolecule Nanodistribution
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F22%3A00077665" target="_blank" >RIV/00159816:_____/22:00077665 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/22:00127154
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acsbiomaterials.2c00650" target="_blank" >https://pubs.acs.org/doi/10.1021/acsbiomaterials.2c00650</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsbiomaterials.2c00650" target="_blank" >10.1021/acsbiomaterials.2c00650</a>
Alternative languages
Result language
angličtina
Original language name
Geometric Control of Cell Behavior by Biomolecule Nanodistribution
Original language description
Many dynamic interactions within the cell micro-environment modulate cell behavior and cell fate. However, the pathways and mechanisms behind cell-cell or cell-extracellular matrix interactions remain understudied, as they occur at a nanoscale level. Recent progress in nanotechnology allows for mimicking of the microenvironment at nanoscale in vitro; electron-beam lithography (EBL) is currently the most promising technique. Although this nanopatterning technique can generate nanostructures of good quality and resolution, it has resulted, thus far, in the production of only simple shapes (e.g., rectangles) over a relatively small area (100 x 100 mu m), leaving its potential in biological applications unfulfilled. Here, we used EBL for cell-interaction studies by coating cell-culture-relevant material with electron-conductive indium tin oxide, which formed nanopatterns of complex nanohexagonal structures over a large area (500 x 500 mu m). We confirmed the potential of EBL for use in cell-interaction studies by analyzing specific cell responses toward differentially distributed nanohexagons spaced at 1000, 500, and 250 nm. We found that our optimized technique of EBL with HaloTags enabled the investigation of broad changes to a cell-culture-relevant surface and can provide an understanding of cellular signaling mechanisms at a single-molecule level.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20900 - Industrial biotechnology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Biomaterials Science & Engineering
ISSN
2373-9878
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
4789-4806
UT code for WoS article
000892436600001
EID of the result in the Scopus database
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