Effect of sarcosine on antioxidant parameters and metallothionein content in the PC-3 prostate cancer cell line
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00068801" target="_blank" >RIV/00216224:14110/13:00068801 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/13:10209746 RIV/00216305:26620/13:PU104424 RIV/00064203:_____/13:10209746 RIV/62156489:43210/13:43907731
Result on the web
<a href="http://dx.doi.org/10.3892/or.2013.2389" target="_blank" >http://dx.doi.org/10.3892/or.2013.2389</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/or.2013.2389" target="_blank" >10.3892/or.2013.2389</a>
Alternative languages
Result language
angličtina
Original language name
Effect of sarcosine on antioxidant parameters and metallothionein content in the PC-3 prostate cancer cell line
Original language description
Sarcosine is currently one of the most discussed markers of prostate cancer. It is involved in amino acid metabolism and methylation processes that occur during the progression of prostate cancer. In this study, we monitored the effect of the addition ofsarcosine (0; 10; 250; 500; 1,000 and 1,500 mu M) in a time-dependent manner (0-72 h) on the PC-3 prostate cancer cell line. For the assessment of cell viability, the commonly used MTT test was employed. Furthermore, ion-exchange liquid chromatography was used for the determination of sarcosine content in the PC-3 cells. We also determined metallothionein (MT) levels by chip capillary electrophoresis and Brdicka reaction in the cells treated with sarcosine. Sarcosine levels in the cells increased in aconcentration-dependent manner levels increased from only 270 nM with the lowest applied concentration of sarcosine (10 mu M) to 106 mu M with the highest applied concentration of sarcosine (1,500 mu M).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Oncology Reports
ISSN
1021-335X
e-ISSN
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Volume of the periodical
29
Issue of the periodical within the volume
6
Country of publishing house
GR - GREECE
Number of pages
8
Pages from-to
2459-2466
UT code for WoS article
000319232800052
EID of the result in the Scopus database
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