Catalytic mechanism for Renilla-type luciferases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F23%3A00079733" target="_blank" >RIV/00159816:_____/23:00079733 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/23:00130829 RIV/00216305:26620/23:PU148240
Result on the web
<a href="https://www.nature.com/articles/s41929-022-00895-z" target="_blank" >https://www.nature.com/articles/s41929-022-00895-z</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41929-022-00895-z" target="_blank" >10.1038/s41929-022-00895-z</a>
Alternative languages
Result language
angličtina
Original language name
Catalytic mechanism for Renilla-type luciferases
Original language description
The widely used coelenterazine-powered Renilla luciferase was discovered over 40 years ago, but the oxidative mechanism by which it generates blue photons remains unclear. Here we decipher Renilla-type catalysis through crystallographic, spectroscopic and computational experiments. Structures of ancestral and extant luciferases complexed with the substrate-like analogue azacoelenterazine or a reaction product were obtained, providing molecular snapshots of coelenterazine-to-coelenteramide oxidation. Bound coelenterazine adopts a Y-shaped conformation, enabling the deprotonated imidazopyrazinone component to attack O-2 via a radical charge-transfer mechanism. A high emission intensity is secured by an aspartate from a conserved proton-relay system, which protonates the excited coelenteramide product. Another aspartate on the rim of the catalytic pocket fine-tunes the electronic state of coelenteramide and promotes the formation of the blue light-emitting phenolate anion. The results obtained also reveal structural features distinguishing flash-type from glow-type bioluminescence, providing insights that will guide the engineering of next-generation luciferase-luciferin pairs for ultrasensitive optical bioassays.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10403 - Physical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nature Catalysis
ISSN
2520-1158
e-ISSN
2520-1158
Volume of the periodical
6
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
16
Pages from-to
23-38
UT code for WoS article
000906600100001
EID of the result in the Scopus database
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