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Plastic response of macrophages to metal ions and nanoparticles in time mimicking metal implant body environment

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F24%3A00079137" target="_blank" >RIV/00159816:_____/24:00079137 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14110/24:00135299 RIV/65269705:_____/24:00079137

  • Result on the web

    <a href="https://link.springer.com/article/10.1007/s11356-023-31430-7" target="_blank" >https://link.springer.com/article/10.1007/s11356-023-31430-7</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11356-023-31430-7" target="_blank" >10.1007/s11356-023-31430-7</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Plastic response of macrophages to metal ions and nanoparticles in time mimicking metal implant body environment

  • Original language description

    The paradigm of using metal biomaterials could be viewed from two sides - treatment of wide spectrum of degenerative diseases, and debris release from materials. After implant insertion, metal nanoparticles (NPs) and ions are released not only upon the first contact with cells/tissues, but in continual manner, which is immediately recognized by immune cells. In this work, the effects of metal nanoparticles (TiO2, Ni) and ions (Ni2+, Co2+, Cr3+, Mo6+) on primary human M0 macrophages from the blood samples of osteoarthritic patients undergoing total arthroplasty were studied in order to monitor immunomodulatory effects on the cells in a real-time format. The highest NiNPs concentration of 10 mu g/ml had no effect on any of macrophage parameters, while the Ni2+ ions cytotoxicity limit for the cells is 0.5 mM. The cytotoxic effects of higher Ni2+ concentration revealed mitochondrial network fragmentation leading to mitochondrial dysfunction, accompanied by increased lysosomal activity and changes in pro-apoptotic markers. The suppression of M2 cell formation ability was connected to presence of Ni2+ ions (0.5 mM) and TiO2NPs (10 mu g/ml). The immunomodulatory effect of Mo6+ ions, controversially, inhibit the formation of the cells with M1 phenotype and potentiate the thread-like shape M2s with increased chaotic cell movement. To summarize, metal toxicity depends on the debris form. Both, metal ions and nanoparticles affect macrophage size, morphological and functional parameters, but the effect of ions is more complex and likely more harmful, which has potential impact on healing and determines post-implantation reactions.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10511 - Environmental sciences (social aspects to be 5.7)

Result continuities

  • Project

    <a href="/en/project/NU20-08-00149" target="_blank" >NU20-08-00149: Multicentric evaluation of hypersensitivity reactions in patients indicated for total joint replacement including evaluation of the reasons for reimplanting</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2024

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Environmental Science and Pollution Research

  • ISSN

    0944-1344

  • e-ISSN

    1614-7499

  • Volume of the periodical

    31

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    19

  • Pages from-to

    4111-4129

  • UT code for WoS article

    001125959900001

  • EID of the result in the Scopus database

    2-s2.0-85182954892