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Diclofenac-Induced Cytotoxicity in Cultured Carp Leukocytes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F20%3A43919373" target="_blank" >RIV/62156489:43210/20:43919373 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14310/20:00118436 RIV/62157124:16270/20:43879041

  • Result on the web

    <a href="https://doi.org/10.33549/physiolres.934609" target="_blank" >https://doi.org/10.33549/physiolres.934609</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.33549/physiolres.934609" target="_blank" >10.33549/physiolres.934609</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Diclofenac-Induced Cytotoxicity in Cultured Carp Leukocytes

  • Original language description

    Diclofenac is a drug commonly used in human and veterinary medicine for the treatment of diseases associated with inflammation and pain. Medicinal products enter waste and surface waters on an everyday basis and contaminate the aquatic environment. Fish are therefore permanently exposed to these chemicals dissolved in their aquatic environment. To simulate variable environmental conditions, the aim of our study was to examine adverse effects of diclofenac under different temperatures of cell incubation (18, 21, 24, 27 and 30 degrees C). Cytotoxic and -static effects of diclofenac in concentrations of 0.001 mu g/ml, 0.01 mu g/ml, 0.1 mu g/ml, 1 mu g/ml, 10 mu g/ml and 100 mu g/ml for the carp (Cyprinus carpio) cultured leukocytes were quantified using detection of lactate dehydrogenase released from damaged cells. Overall DCF cytotoxicity was relatively low and its impact was pronounced at higher temperature and DCF concentration. Cells growth inhibition is changing more rapidly but it is high mainly at the highest concentration from low temperature. DNA fragmentation was not detected in tested leukocyte cell line. CYP450 increased diclofenac cytotoxicity only at the highest concentration but at incubation temperatures 18 and 27 degrees C. Leukocyte viability is essential for immune functions and any change can lead to reduction of resistance against pathogens, mainly in cold year seasons, when the immune system is naturally suppressed.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    40103 - Fishery

Result continuities

  • Project

    <a href="/en/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Sustainable production of healthy fish in various aquaculture systems - PROFISH</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Physiological Research

  • ISSN

    0862-8408

  • e-ISSN

  • Volume of the periodical

    69

  • Issue of the periodical within the volume

    Supplement 4

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    12

  • Pages from-to

    "S607"-"S618"

  • UT code for WoS article

    000621838200007

  • EID of the result in the Scopus database

    2-s2.0-85102229899