Diclofenac-induced cytotoxicity in cultured carp leukocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081766%3A_____%2F20%3A00540434" target="_blank" >RIV/68081766:_____/20:00540434 - isvavai.cz</a>
Result on the web
<a href="https://www.biomed.cas.cz/physiolres/pdf/2020/69_S607.pdf" target="_blank" >https://www.biomed.cas.cz/physiolres/pdf/2020/69_S607.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.33549/physiolres.934609" target="_blank" >10.33549/physiolres.934609</a>
Alternative languages
Result language
angličtina
Original language name
Diclofenac-induced cytotoxicity in cultured carp leukocytes
Original language description
Diclofenac is a drug commonly used in human and veterinary medicine for the treatment of diseases associated with inflammation and pain. Medicinal products enter waste and surface waters on an everyday basis and contaminate the aquatic environment. Fish are therefore permanently exposed to these chemicals dissolved in their aquatic environment. To simulate variable environmental conditions, the aim of our study was to examine adverse effects of diclofenac under different temperatures of cell incubation (18, 21, 24, 27 and 30 °C). Cytotoxic and -static effects of diclofenac in concentrations of 0.001 μg/ml, 0.01 μg/ml, 0.1 μg/ml, 1 μg/ml, 10 μg/ml and 100 μg/ml for the carp (Cyprinus carpio) cultured leukocytes were quantified using detection of lactate dehydrogenase released from damaged cells. Overall DCF cytotoxicity was relatively low and its impact was pronounced at higher temperature and DCF concentration. Cells growth inhibition is changing more rapidly but it is high mainly at the highest concentration from low temperature. DNA fragmentation was not detected in tested leukocyte cell line. CYP450 increased diclofenac cytotoxicity only at the highest concentration but at incubation temperatures 18 and 27 °C. Leukocyte viability is essential for immune functions and any change can lead to reduction of resistance against pathogens, mainly in cold year seasons, when the immune system is naturally suppressed.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiological Research
ISSN
1802-9973
e-ISSN
—
Volume of the periodical
69
Issue of the periodical within the volume
Supplement 4
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
12
Pages from-to
"S607"-"S618"
UT code for WoS article
000621838200007
EID of the result in the Scopus database
2-s2.0-85102229899