Production of Cytokines from Bovine Mammary Gland Leukocytes Influenced by Bacterial Infection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43909518" target="_blank" >RIV/62156489:43210/16:43909518 - isvavai.cz</a>
Result on the web
<a href="https://sites.google.com/site/physiologyanimal2016/home/prispevky" target="_blank" >https://sites.google.com/site/physiologyanimal2016/home/prispevky</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Production of Cytokines from Bovine Mammary Gland Leukocytes Influenced by Bacterial Infection
Original language description
The objective of this trial was to study the inflammatory cytokines, which are produced by bovine mammary gland leukocytes. The leukocytes were obtained by mammary gland lavage and incubated for 1, 2 and 18 hours with lipopolysaccharide (50 μg/ml) or with muramyldipeptide (500 μg/ml) in vitro. LPS was used as a toxin of Gram-negative bacteria, and muramyldipeptide as a structural unit of a peptidoglycan of Gram-positive bacteria. The concentration levels of these cytokines were analyzed by ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. The IL-1β concentration level was higher for lipopolysacharide incubation than for muramyldipeptide incubation. The production of IL- 1β started 2 hours following the cell incubation with lipopolysacharide. The production of IL- 1β started 18 hours following the cell incubation with muramyldipeptide. The production of IL-4 started 1, 2, and 18 hours following the cell incubation with both bacteria components. Higher IL-4 concentration was recorded for lipopolysacharide incubation.
Czech name
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Czech description
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Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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