Production of Interferon Gamma and Transforming Growth Factor Beta 1 from Bovine Mammary Gland Leukocytes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43909255" target="_blank" >RIV/62156489:43210/17:43909255 - isvavai.cz</a>
Result on the web
<a href="http://docsdrive.com/pdfs/medwelljournals/javaa/2017/119-121.pdf" target="_blank" >http://docsdrive.com/pdfs/medwelljournals/javaa/2017/119-121.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Production of Interferon Gamma and Transforming Growth Factor Beta 1 from Bovine Mammary Gland Leukocytes
Original language description
The objective of this trial was to study the inflammatory cytokines interferon gamma (IFN-γ) and transforming growth factor beta 1 (TGF-β1) which are produced by bovine mammary gland leukocytes. The leukocytes were obtained by mammary gland lavage and incubated for 1, 2 and 18 hours with lipopolysaccharide (50 µg/ml) or with muramyl dipeptide (500 µg/ml) in vitro. Lipopolyccharide was used as a toxin of Gram-negative bacteria, and muramyl dipeptide as a structural unit of a peptidoglycan of Gram-positive bacteria. The concentration levels of these cytokines were analyzed by ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. Higher IFN-γ concentration was recorded for lipopolysacharide incubation. The production of IFN-γ started 18 hours following the cell incubation with lipopolysaccharide or with muramyl dipeptide. The production of TGF-β1 was higher for lipopolysaccharide incubation than for muramyl dipeptide incubation throughout the all set time points. The TGF-β1 production started 2 hours following the cell incubation with muramyl dipeptide.
Czech name
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Czech description
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Classification
Type
J<sub>ost</sub> - Miscellaneous article in a specialist periodical
CEP classification
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OECD FORD branch
40301 - Veterinary science
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Animal and Veterinary Advances
ISSN
1680-5593
e-ISSN
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Volume of the periodical
16
Issue of the periodical within the volume
8
Country of publishing house
PK - PAKISTAN
Number of pages
3
Pages from-to
119-121
UT code for WoS article
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EID of the result in the Scopus database
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