TGF-beta 1 production during experimentally induced mastitis caused by lipopolysaccharide

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912841" target="_blank" >RIV/62156489:43210/17:43912841 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

TGF-beta 1 production during experimentally induced mastitis caused by lipopolysaccharide

Original language description

Lipopolysaccharide is an endotoxin released from the cell wall of Gram-negative bacteria. Infusion of lipopolysaccha- ride into the bovine mammary gland induces an inflamma-tory response [1]. Inflammation of the mammary gland is accompanied by the production of inflammatory cytokines [2,3]. Transforming growth factor beta 1 (TGF-b1) is produced in very high levels by platelets. Other types of cells which produce this cytokine are macrophages, lymphocytes, endothelial cells and fibroblasts. The aim of this study was to evaluate concentration of TGF-b1 in course of in vivo experimentally induced bovine mastitis by lipopolysaccha- ride. The experiments were carried out on the mammary glands of virgin heifers. TGF-b1 concentration was quan-tified by ELISA in four time points following stimulation of mammary glands by lipopolysaccharide (24, 48, 72, 168 hours). The maximum of TGF-b1 concentration was measured 48 hours following intramammary injection of lipopolysaccharide. It seems to be that 48 hours is the time to start regeneration of damaged tissues because TGF-b1is the cytokine responsible for wound healing.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů