The effect of peptidoglycan on production of pro-inflammatory cytokines by mammary gland leukocytes during in vitro study
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914682" target="_blank" >RIV/62156489:43210/18:43914682 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2018/mnet_2018_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The effect of peptidoglycan on production of pro-inflammatory cytokines by mammary gland leukocytes during in vitro study
Original language description
Cytokine network is very complicated and new knowledge about these proteins is important to develop better system of prevention, diagnosis and therapy of mastitis including decrease of antibiotic treatment. The study was implemented on eight selected healthy heifers. Leukocytes were obtained by lavage of the mammary gland 24 hours following the mammary gland stimulation by phosphate buffered saline solution (PBS). Leukocytes were cultivated for three different times. The first were cultivated for one hour, next two hours and eighteen hours. For cultivating was using peptidoglycan. Concentration of IL-1β and IFN-γ was measured by ELISA. IL-1β has the higher concentration after one hour cultivation and IFN-γ after two hours cultivation. Stimulation of mammary gland leukocytes with peptidoglycan resulted in a significant increase in the concentration of cytokines in comparison to the control. The concentration of IL-1β peaked at 1-hour incubation. In 2 and 18 hours, there were found decreasing of the concentration of this cytokine.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2018: Proceedings of International PhD Students Conference
ISBN
978-80-7509-597-8
ISSN
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e-ISSN
neuvedeno
Number of pages
4
Pages from-to
388-391
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 7, 2018
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000462205300073