Production of interleukin 1beta and interferon gamma by bovine mammary gland leukocytes stimulated by lipopolysaccharide
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43914684" target="_blank" >RIV/62156489:43210/18:43914684 - isvavai.cz</a>
Result on the web
<a href="https://dx.doi.org/10.15414/jmbfs.2018-19.8.3.882-884" target="_blank" >https://dx.doi.org/10.15414/jmbfs.2018-19.8.3.882-884</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.15414/jmbfs.2018-19.8.3.882-884" target="_blank" >10.15414/jmbfs.2018-19.8.3.882-884</a>
Alternative languages
Result language
angličtina
Original language name
Production of interleukin 1beta and interferon gamma by bovine mammary gland leukocytes stimulated by lipopolysaccharide
Original language description
The aim of this trial was to investigate the production of the pro-inflammatory cytokines interleukin 1beta (IL-1β) and interferon gamma (IFN-γ) produced by bovine mammary gland leukocytes following lipopolysaccharide (LPS) challenge (5 μg LPS of Escherichia coliin 20 ml of PBS) or PBS (20 ml) as a control. The leukocytes were obtained by mammary gland lavage 1, 2, 3, and 7 days following the stimulation. The concentration levels of these cytokines were analyzed by sandwich ELISA. Eight clinically healthy crossbred heifers (Holstein x Czech Pied) were selected for this study. The heifers were group housed in a tie-stall barn and fed a total mixed diet. Higher average number of leukocytes (P<0.01) was recorded for LPS than for PBS stimulation on day 1. The production of IL-1β increased following the stimulation on day 1. After that gradual decline was detected. The IFN-γ production showed a similar trend. Positive correlation between IL-1β and IFN-γ increased level was found.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40201 - Animal and dairy science; (Animal biotechnology to be 4.4)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Microbiology, Biotechnology and Food Science
ISSN
1338-5178
e-ISSN
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Volume of the periodical
8
Issue of the periodical within the volume
3
Country of publishing house
SK - SLOVAKIA
Number of pages
3
Pages from-to
882-884
UT code for WoS article
000455221000005
EID of the result in the Scopus database
2-s2.0-85064629841