Inflammatory cytokines produced by leukocytes of bovine mammary gland
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912601" target="_blank" >RIV/62156489:43210/17:43912601 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Inflammatory cytokines produced by leukocytes of bovine mammary gland
Original language description
The aim of this work was to study inflammatory cytokines - the tumor necrosis factor alpha (TNF-α) and the interleukin 10 (IL-10), which are produced by bovine mammary gland leukocytes. Eight clinically healthy heifers were selected for this study. Obtained leukocytes were incubated for 1, 2 and 18 hours with lipopolysaccharide (LPS) or muramyldipeptide (MDP) under in vitro conditions. Concentration of TNF-α and IL-10 was measured by ELISA method. Production of TNF-α by leukocytes of bovine mammary gland was higher (LPS:552.57 pg/ml [+-27.35]; MDP:680.20 pg/ml [+-31.32]) than production of TNF-α by blood leukocytes (LPS:344.87 pg/ml [+-23.12]; MDP:285.56 pg/ml [+-19.87]). Concentration of TNF-α was increased after 18 hours of incubation. IL-10 production by bovine mammary gland leukocytes was higher (LPS:26.02 u/ml [+-3.44] and 36.98 u/ml [+-7.78]; MDP:22.33 u/ml [+-3.02] and 33.45 u/ml [+-8.28]) than production of IL-10 by blood leukocytes (LPS:12.67 u/ml [+-2.47]; MDP:9.45 u/ml [+-3.61]). IL-10 concentration of bovine mammary gland leukocytes was increased after 2 hours of incubation. Differential blood leukocyte count was determined by method of light microscopy.
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
40301 - Veterinary science
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2017: Proceedings of International PhD Students Conference
ISBN
978-80-7509-529-9
ISSN
—
e-ISSN
neuvedeno
Number of pages
5
Pages from-to
708-712
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 8, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000440194500126