Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F24%3A00081022" target="_blank" >RIV/00159816:_____/24:00081022 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/24:00135617
Result on the web
<a href="https://www.sciencedirect.com/science/article/abs/pii/S2772950824000621" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S2772950824000621</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bioadv.2024.213819" target="_blank" >10.1016/j.bioadv.2024.213819</a>
Alternative languages
Result language
angličtina
Original language name
Hyaluronic acid-based hydrogels with tunable mechanics improved structural and contractile properties of cells
Original language description
Extracellular matrix (ECM) regulates cellular responses through mechanotransduction. The standard approach of in vitro culturing on plastic surfaces overlooks this phenomenon, so there is a need for biocompatible materials that exhibit adjustable mechanical and structural properties, promote cell adhesion and proliferation at low cost and for use in 2D or 3D cell cultures. This study presents a new tunable hydrogel system prepared from highmolecular hyaluronic acid (HA), Bovine serum albumin (BSA), and gelatin cross-linked using EDC/NHS. Hydrogels with Young's moduli (E) ranging from subunit to units of kilopascals were prepared by gradually increasing HA and BSA concentrations. Concentrated high-molecular HA network led to stiffer hydrogel with lower cluster size and swelling capacity. Medium and oxygen diffusion capability of all hydrogels showed they are suitable for 3D cell cultures. Mechanical and structural changes of mouse embryonic fibroblasts (MEFs) on hydrogels were compared with cells on standard cultivation surfaces. Experiments showed that hydrogels have suitable mechanical and cell adhesion capabilities, resulting in structural changes of actin filaments. Lastly, applying hydrogel for a more complex HL-1 cell line revealed improved mechanical and electrophysiological contractile properties.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10601 - Cell biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biomaterials Advances
ISSN
2772-9508
e-ISSN
2772-9508
Volume of the periodical
159
Issue of the periodical within the volume
MAY 2024
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
12
Pages from-to
213819
UT code for WoS article
001218651500001
EID of the result in the Scopus database
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