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COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS AS BETTER SURFACE FOR CELL CULTURE

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F21%3A00075912" target="_blank" >RIV/00159816:_____/21:00075912 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216224:14740/21:00124449

  • Result on the web

    <a href="http://dx.doi.org/10.37904/nanocon.2020.3739" target="_blank" >http://dx.doi.org/10.37904/nanocon.2020.3739</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.37904/nanocon.2020.3739" target="_blank" >10.37904/nanocon.2020.3739</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    COVALENTLY CROSS-LINKED HYALURONIC ACID/BSA/GELATINE HYDROGELS AS BETTER SURFACE FOR CELL CULTURE

  • Original language description

    The mechanical and biomechanical properties of the cell&apos;s extracellular matrix is a factor in their later development. In this regard, elastic biocompatible materials as a surface for cultivating cells are in recent years, becoming an exciting field of study. Hydrogels are a hydrophilic 3D network of synthetic polymers or biopolymers. They can have tunable mechanical properties, controllable degradability, or they can be designed for long-time release of treatments. Here, hydrogels from Hyaluronic acid (HA), Bovine serum albumin (BSA) and gelatin was prepared, via carbodiimide chemistry. Morphology and mechanical properties of these hydrogels was characterized via Atomic Force Microscopy (AFM). Young&apos;s modulus of HA/BSA and HA/BSA/gel were 124.7 +/- 15.9 and 20.3 +/- 0.5 kPa, respectively. Mouse embryonic fibroblasts were then cultivated on hydrogels and on the glass surface as a reference. Mechanical and morphological properties of fibroblasts were examined by AFM and via phalloidin-TRITC staying. Relationship between the structure of cells and the type of surface was seen. Cells grown on glass were more flattened with an evenly linear actin structure. On hydrogels, fibroblasts were more star-shaped with non-linear actin structures and many filopodia. Furthermore, height of cells was higher was lower compared to cells on both types of hydrogels (0.36 mu m vs. 0.56 mu m vs. 1.03 mu m). Young&apos;s modulus of cells on fibroblast was highest, which relates to the whole range of structural changes linked with surface stiffness. In conclusion, this type of hydrogel might be utilized in the future as a better surface material for cell cultivation.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    12TH INTERNATIONAL CONFERENCE ON NANOMATERIALS - RESEARCH &amp; APPLICATION (NANOCON 2020)

  • ISBN

    978-80-87294-98-7

  • ISSN

  • e-ISSN

  • Number of pages

    7

  • Pages from-to

    366-372

  • Publisher name

    TANGER LTD

  • Place of publication

    SLEZSKA

  • Event location

    Brno

  • Event date

    Oct 21, 2020

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article

    000664505500062