Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F25%3A00082314" target="_blank" >RIV/00159816:_____/25:00082314 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/25:00141583
Result on the web
<a href="https://academic.oup.com/bbb/article-abstract/89/6/898/8052007" target="_blank" >https://academic.oup.com/bbb/article-abstract/89/6/898/8052007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/bbb/zbaf022" target="_blank" >10.1093/bbb/zbaf022</a>
Alternative languages
Result language
angličtina
Original language name
Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization
Original language description
Haloalkane dehalogenases (HLDs) convert halogenated compounds to corresponding alcohols by a simple hydrolytic mechanism. Although many bacterial strains possess HLDs or HLD homologues, LinB is the only HLD known to be involved in the utilization of gamma-hexachlorocyclohexane (gamma-HCH), a man-made chlorinated pesticide. In this study, to gain insight into the functional evolution of HLDs toward gamma-HCH utilization, the linB gene in gamma-HCH-degrading Sphingobium japonicum strain UT26 was replaced by 7 other HLD or HLD homologous genes, including 3 putative ancestral enzymes. Interestingly, strains carrying genes for DmmA from a marine metagenome or Rluc_anc, an ancestor of Renilla-luciferin 2-monooxygenase and LinB, produced 2,5-dichlorophenol and 2,5-dichlorohydroquinone from gamma-HCH, which are indicators of LinB activity, and grew in minimal medium supplied with gamma-HCH as a sole carbon source. These results indicated that other HLDs, in addition to LinB, can play roles in the gamma-HCH utilization.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN
0916-8451
e-ISSN
1347-6947
Volume of the periodical
89
Issue of the periodical within the volume
6
Country of publishing house
JP - JAPAN
Number of pages
12
Pages from-to
898-909
UT code for WoS article
001457346000001
EID of the result in the Scopus database
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