Sequence diversity in haloalkane dehalogenases, as revealed by PCR using family-specific primers

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00378920" target="_blank" >RIV/61388971:_____/12:00378920 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.mimet.2011.11.013" target="_blank" >http://dx.doi.org/10.1016/j.mimet.2011.11.013</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.mimet.2011.11.013" target="_blank" >10.1016/j.mimet.2011.11.013</a>

Result language
angličtina
Original language name
Sequence diversity in haloalkane dehalogenases, as revealed by PCR using family-specific primers
Original language description
Haloalkane dehalogenases (HLDs) are hydrolytic enzymes that cleave carbon-halogen bonds in various halogenated compounds. Interest initially grew in HLDs as biocatalysts for bioremediation and later for biotransformation applications; each specific HLD within the HLD family has its own substrate specificity, enantioselectivity and product inhibition characteristics. We developed degenerate oligonucleotide primers for HLD-encoding genes and used these to PCR-amplify large hid gene fragments using genomicDNA from the microbial community of a chlorinated-solvent-contaminated aquifer as a template. An analysis of small subunit ribosomal RNA genes revealed a high complexity in the eubacterial population, dominated by alpha-, beta- and gamma-Proteobacteria,and Acidobacteria
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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