Methylation analysis of tumor suppressor genes in endometroid carcinoma of endometrium using MS-MLPA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F13%3A10191974" target="_blank" >RIV/00179906:_____/13:10191974 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/13:10191974

Result on the web

<a href="http://biomed.papers.upol.cz/pdfs/bio/2013/04/05.pdf" target="_blank" >http://biomed.papers.upol.cz/pdfs/bio/2013/04/05.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5507/bp.2013.035" target="_blank" >10.5507/bp.2013.035</a>

Result language

angličtina

Original language name

Methylation analysis of tumor suppressor genes in endometroid carcinoma of endometrium using MS-MLPA

Original language description

Background. Epigenetic changes are considered to be a frequent event during tumor development. Hypermethylation of promoter CpG islands represents an alternative mechanism for inactivation of tumor suppressor genes, DNA repair genes, cell cycle regulators and transcription factors. The aim of this study was to investigate promoter methylation of specific genes in endometrial cancer by comparison with normal endometrial tissue. Materials and Methods. We used MS-MLPA (Methylation-specific Multiplex ligation-dependent probe amplification) to compare the methylation status of 59 tissue samples of endometroid type of endometrial carcinoma with 20 control samples of non-neoplastic endometrium. Results. Using 15% cut-off for methylation, we observed significantly higher methylation in the CDH13 gene in endometrial cancer group. We observed significantly higher methylation in both WT1 and GATA5 genes in IB stage of endometroid carcinoma. We also observed significantly higher methylation in GAT

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FK - Gynaecology and obstetrics

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Papers

ISSN

1213-8118

e-ISSN

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Volume of the periodical

157

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

298-303

UT code for WoS article

000329091500006

EID of the result in the Scopus database

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