Interaction of cholinesterase modulators with DNA and their cytotoxic activity
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F14%3A10209873" target="_blank" >RIV/00179906:_____/14:10209873 - isvavai.cz</a>
Alternative codes found
RIV/62690094:18470/14:50002324
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0141813013006314" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0141813013006314</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijbiomac.2013.11.022" target="_blank" >10.1016/j.ijbiomac.2013.11.022</a>
Alternative languages
Result language
angličtina
Original language name
Interaction of cholinesterase modulators with DNA and their cytotoxic activity
Original language description
This research was focused on a study of the binding properties of a series of cholinesterase reactiva-tors compounds K075 (1), K027 (2) and inhibitors compounds K524, K009 and 7-MEOTA (3-5) with calfthymus DNA. The nature of the interactions between compounds 1-5 and DNA were studied using spec-troscopic techniques (UV-vis, fluorescence spectroscopy and circular dichroism). The binding constantsfor complexes of cholinesterase modulators with DNA were determined from UV-vis spectroscopic titra-tions (K =0.5 x 104-8.9 x 105MMINUS SIGN 1). The ability of the prepared analogues to relax topoisomerase I wasstudied with electrophoretic techniques and it was proved that ligands 4 and 5 inhibited this enzymeat a concentration of 30 M. The biological activityof the novel compounds was assessed through anexamination of changes in cell cycle distribution, mitochondrial membrane potential and cellular via-bility. Inhibitors 3-5 exhibited a cytotoxic effect on HL-60 (human acute promyelocytic leu
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Biological Macromolecules
ISSN
0141-8130
e-ISSN
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Volume of the periodical
64
Issue of the periodical within the volume
March
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
53-62
UT code for WoS article
000334147300009
EID of the result in the Scopus database
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