Detection panel for identification of twelve hemorrhagic viruses using real-time RT-PCR

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F14%3A10284347" target="_blank" >RIV/00179906:_____/14:10284347 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/14:00439429 RIV/00027162:_____/14:#0001164 RIV/60162694:G44__/14:43875371

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detection panel for identification of twelve hemorrhagic viruses using real-time RT-PCR

Original language description

Background: Viral hemorrhagic fevers are caused by viruses from four viral families and develop diseases with high fatality rates. However, no commercial diagnostic assay for these pathogens is available. Findings: We developed real-time RT-PCR assays for viruses Ebola, Marburg, Lassa, Guanarito, Machupo, Junin, Sabiá, Seoul, Puumala, Hantaan, Crimean-Congo hemorrhagic fever virus and Rift Valley fever virus. The assays were optimized for identical reaction conditions and can be performed using severaltypes of real-time PCR instruments, both capillary and plate, including a portable Ruggedized Advanced Pathogen Identification Device (R.A.P.I.D.) (Idaho Technology, Inc.). Conclusions: In combination with primers and probes from previously published studies, we present a simple system for rapid identification of hemorrhagic filoviruses, arenaviruses and bunyaviruses with sufficient sensitivity for first contact laboratory and diagnosis under field conditions.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Epidemiologie, Mikrobiologie, Imunologie

ISSN

1210-7913

e-ISSN

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Volume of the periodical

63

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

238-244

UT code for WoS article

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EID of the result in the Scopus database

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