Natural Detoxification Capacity to Inactivate Nerve Agents Sarin and VX in the Rat Blood

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F15%3A10321940" target="_blank" >RIV/00179906:_____/15:10321940 - isvavai.cz</a>

Alternative codes found

RIV/60162694:G44__/15:43875561

Result on the web

<a href="http://actamedica.lfhk.cuni.cz/media/pdf/am_2015058040128.pdf" target="_blank" >http://actamedica.lfhk.cuni.cz/media/pdf/am_2015058040128.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14712/18059694.2016.4" target="_blank" >10.14712/18059694.2016.4</a>

Result language

angličtina

Original language name

Natural Detoxification Capacity to Inactivate Nerve Agents Sarin and VX in the Rat Blood

Original language description

Background: The method of continual determination of the rat blood cholinesterase activity was developed to study the changes of the blood cholinesterases following different intervetions. Aims: The aim of this study is registration of cholinesterase activity in the rat blood and its changes to demonstrate detoxification capacity of rats to inactivate sarin or VX in vivo. Methods: The groups of female rats were premedicated (ketamine and xylazine) and cannulated to a. femoralis. Continual blood sampling (0.02 ml/min) and monitoring of the circulating blood cholinesterase activity were performed. Normal activity was monitored 1-2 min and then the nerve agent was administered i.m. (2x LD50). Using different time intervals of the leg compression and relaxation following the agent injection, cholinesterase activity was monitored and according to the inhibition obtained, detoxification capacitywas assessed. Results: Administration of sarin to the leg, then 1 and 5 min compression and 20 min later relaxation showed that further inhibition in the blood was not observed. On the other hand, VX was able to inhibit blood cholinesterases after this intervention. Conclusions: The results demonstrated that sarin can be naturally detoxified on the contrary to VX. Described method can be used as model for other studies dealing with changes of cholinesterases in the blood following different factors.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Medica (Hradec Králové)

ISSN

1211-4286

e-ISSN

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Volume of the periodical

58

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

3

Pages from-to

128-130

UT code for WoS article

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EID of the result in the Scopus database

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