Longitudinal molecular characterization of endoscopic specimens from colorectal lesions
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F16%3A10327248" target="_blank" >RIV/00179906:_____/16:10327248 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/16:10327248 RIV/00216208:11150/16:10327248 RIV/61383082:_____/16:00000203
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873886/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873886/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3748/wjg.v22.i20.4936" target="_blank" >10.3748/wjg.v22.i20.4936</a>
Alternative languages
Result language
angličtina
Original language name
Longitudinal molecular characterization of endoscopic specimens from colorectal lesions
Original language description
AIM: To compare molecular profiles of proximal colon, distal colon and rectum in large adenomas, early and late carcinomas. To assess feasibility of testing directed at molecular markers from this study in routine clinical practice. METHODS: A prospective 3-year study has resulted in the acquisition of samples from 159 large adenomas and 138 carcinomas along with associated clinical parameters including localization, grade and histological type for adenomas and localization and stage for carcinomas. A complex molecular phenotyping has been performed using multiplex ligation-dependent probe amplification technique for the evaluation of CpG-island methylator phenotype (CIMP), PCR fragment analysis for detection of microsatellite instability and denaturing capillary electrophoresis for sensitive detection of somatic mutations in KRAS, BRAF, TP53 and APC genes. RESULTS: Molecular types according to previously introduced Jass classification have been evaluated for large adenomas and early and late carcinomas. An increase in CIMP+ type, eventually accompanied with KRAS mutations, was notable between large adenomas and early carcinomas. As expected, the longitudinal observations revealed a correlation of the CIMP+/BRAF+ type with proximal location. CONCLUSION: Prospective molecular classification of tissue specimens is feasible in routine endoscopy practice. Increased frequency of some molecular types corresponds to the developmental stages of colorectal tumors. As expected, a clear distinction is notable for tumors located in proximal colon supposedly arising from the serrated (methylation) pathway.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FE - Other fields of internal medicine
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/NT14383" target="_blank" >NT14383: Use of cfDNA as a new intention for minimally-invasive diagnosis and precise molecular classification of colorectal tumors.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
World Journal of Gastroenterology
ISSN
1007-9327
e-ISSN
—
Volume of the periodical
22
Issue of the periodical within the volume
20
Country of publishing house
CN - CHINA
Number of pages
10
Pages from-to
4936-4945
UT code for WoS article
000377075500016
EID of the result in the Scopus database
2-s2.0-84979021669