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Longitudinal molecular characterization of endoscopic specimens from colorectal lesions

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F16%3A10327248" target="_blank" >RIV/00179906:_____/16:10327248 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216208:11110/16:10327248 RIV/00216208:11150/16:10327248 RIV/61383082:_____/16:00000203

  • Result on the web

    <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873886/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873886/</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3748/wjg.v22.i20.4936" target="_blank" >10.3748/wjg.v22.i20.4936</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Longitudinal molecular characterization of endoscopic specimens from colorectal lesions

  • Original language description

    AIM: To compare molecular profiles of proximal colon, distal colon and rectum in large adenomas, early and late carcinomas. To assess feasibility of testing directed at molecular markers from this study in routine clinical practice. METHODS: A prospective 3-year study has resulted in the acquisition of samples from 159 large adenomas and 138 carcinomas along with associated clinical parameters including localization, grade and histological type for adenomas and localization and stage for carcinomas. A complex molecular phenotyping has been performed using multiplex ligation-dependent probe amplification technique for the evaluation of CpG-island methylator phenotype (CIMP), PCR fragment analysis for detection of microsatellite instability and denaturing capillary electrophoresis for sensitive detection of somatic mutations in KRAS, BRAF, TP53 and APC genes. RESULTS: Molecular types according to previously introduced Jass classification have been evaluated for large adenomas and early and late carcinomas. An increase in CIMP+ type, eventually accompanied with KRAS mutations, was notable between large adenomas and early carcinomas. As expected, the longitudinal observations revealed a correlation of the CIMP+/BRAF+ type with proximal location. CONCLUSION: Prospective molecular classification of tissue specimens is feasible in routine endoscopy practice. Increased frequency of some molecular types corresponds to the developmental stages of colorectal tumors. As expected, a clear distinction is notable for tumors located in proximal colon supposedly arising from the serrated (methylation) pathway.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    FE - Other fields of internal medicine

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/NT14383" target="_blank" >NT14383: Use of cfDNA as a new intention for minimally-invasive diagnosis and precise molecular classification of colorectal tumors.</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    World Journal of Gastroenterology

  • ISSN

    1007-9327

  • e-ISSN

  • Volume of the periodical

    22

  • Issue of the periodical within the volume

    20

  • Country of publishing house

    CN - CHINA

  • Number of pages

    10

  • Pages from-to

    4936-4945

  • UT code for WoS article

    000377075500016

  • EID of the result in the Scopus database

    2-s2.0-84979021669