Longitudinal molecular characterization of endoscopic specimens from colorectal lesions

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26475821%3A_____%2F16%3AN0000012" target="_blank" >RIV/26475821:_____/16:N0000012 - isvavai.cz</a>

Result on the web

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873886/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4873886/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3748/wjg.v22.i20.4936." target="_blank" >10.3748/wjg.v22.i20.4936.</a>

Result language

angličtina

Original language name

Longitudinal molecular characterization of endoscopic specimens from colorectal lesions

Original language description

A prospective 3-year study has resulted in the acquisition of samples from 159 large adenomas and 138 carcinomas along with associated clinical parameters including localization, grade and histological type for adenomas and localization and stage for carcinomas. A complex molecular phenotyping has been performed using multiplex ligation-dependent probe amplification technique for the evaluation of CpG-island methylator phenotype (CIMP), PCR fragment analysis for detection of microsatellite instability and denaturing capillary electrophoresis for sensitive detection of somatic mutations in KRAS, BRAF, TP53 and APC genes. Molecular types according to previously introduced Jass classification have been evaluated for large adenomas and early and late carcinomas. An increase in CIMP+ type, eventually accompanied with KRAS mutations, was notable between large adenomas and early carcinomas. As expected, the longitudinal observations revealed a correlation of the CIMP+/BRAF+ type with proximal location. Prospective molecular classification of tissue specimens is feasible in routine endoscopy practice. Increased frequency of some molecular types corresponds to the developmental stages of colorectal tumors. As expected, a clear distinction is notable for tumors located in proximal colon supposedly arising from the serrated (methylation) pathway.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/NT14383" target="_blank" >NT14383: Use of cfDNA as a new intention for minimally-invasive diagnosis and precise molecular classification of colorectal tumors.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

World journal of gastroenterology

ISSN

2219-2840

e-ISSN

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Volume of the periodical

22

Issue of the periodical within the volume

20

Country of publishing house

US - UNITED STATES

Number of pages

10

Pages from-to

4936-4945

UT code for WoS article

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EID of the result in the Scopus database

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