DNA/BSA binding ability and genotoxic effect of mono- and binuclear copper (II) complexes containing a Schiff base derived from salicylaldehyde and D, L-glutamic acid
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F17%3A10337623" target="_blank" >RIV/00179906:_____/17:10337623 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jorganchem.2016.11.007" target="_blank" >http://dx.doi.org/10.1016/j.jorganchem.2016.11.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jorganchem.2016.11.007" target="_blank" >10.1016/j.jorganchem.2016.11.007</a>
Alternative languages
Result language
angličtina
Original language name
DNA/BSA binding ability and genotoxic effect of mono- and binuclear copper (II) complexes containing a Schiff base derived from salicylaldehyde and D, L-glutamic acid
Original language description
This study examined a two of Cu(II) complexes containing Schiff bases [Cu(salglu)Meim] (1) and [Cu(et)(2)(im)(4)][Cu-2(salglu)(2)(im)(2)] (2), where salglu = (N-salicylidene-D,L-glutamato)(2-) Meim = 2-methylimidazole, im = imidazole and et = ethanol. The interactions and binding characteristics of the complexes with calf thymus DNA (ctDNA) and bovine serum albumin (BSA) were investigated using UV-Vis and fluorescence spectroscopy. The binding constants K were calculated from the UV-Vis spectroscopic titration results using the McGhee and Hippel equation, and were found to be K = 8.9 (for 1) and 3.1 x 10(3) M-1 (for 2) in the case of complex-DNA interactions, while Stern-Volmer quenching results were used to determine the values K = 2.74 x 10(5) and 2.54 x 10(6) M-1 (for 1 and 2, respectively) in the case of complex-BSA binding. DNA strand breaks in cellular DNA were investigated using single cell gel electrophoresis (comet assay). MCF-7 cancer cell lines and healthy HEK-293T cell lines were exposed to complexes 1 and 2 at a concentration range of 0.001-100 x 10(-6) M for 24 h. Complexes 1 and 2 induced significant DNA damage in MCF-7 cancer cell lines, while healthy HEK-293T cell lines were more resistant against their effect.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Organometallic Chemistry
ISSN
0022-328X
e-ISSN
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Volume of the periodical
827
Issue of the periodical within the volume
January
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
67-77
UT code for WoS article
000389610700008
EID of the result in the Scopus database
2-s2.0-84994651595